Gc rich region calculator
I'm trying to amplify up a very GC rich (87%) region for pyrosequencing PCR. I'm getting lots of non-specific amplification - I've tried varying concentrations of DMSO (10%, 5%, %), betaine (1. GC content: % 1 ml of a sol'n with an Absorbance of at nm is microMolar 5 and contains micrograms. 1 °C (Basic) an online oligonucleotide properties calculator'. () Nucleic Acids Res. 35(webserver issue): May (Abstract/Full text) This page may be freely linked or distributed for any educational or non-commercial use. Choose from a variety of PCR enzymes and reagents for your applications, with the flexibility needed to perform your experiments. With PCR enzymes you know and trust such as Applied Biosystems AmpliTaq and AmpliTaq Gold, and Invitrogen Platinum Taq and Platinum SuperFi DNA polymerases, we have what it takes for successful PCR.